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Journal: bioRxiv
Article Title: Dual effects of nicotinamide on aging-related arrhythmia: protective at low dose, proarrhythmic at high doses
doi: 10.1101/2025.09.22.677785
Figure Lengend Snippet: (A) Experimental scheme of Langendorff ex vivo mouse hearts perfused with control or 10 mM NAM-supplemented buffer. Electrocardiogram (ECG) was recorded with the positive electrode (‘+’) positioned beneath the apex of the left ventricle, and the negative (‘-’) and ground (‘0’) electrodes on the cannula. Programmed electrical stimulation was applied via a pacing electrode (‘P’) placed on the surface of the right atrium. (B) Representative ECG traces from AF-negative (top) and AF-positive (bottom) mouse hearts. (C-E) AF incidence, episode count and burden in hearts from the control (n=6 for both sexes) and 10 mM NAM (n=5 for both sexes). Data are expressed as mean ± standard error of the mean. Chi-square test was used for AF incidence and Scheirer-Ray-Hare test followed by Dunn’s post-hoc test was used for AF episode count and burden.
Article Snippet:
Techniques: Ex Vivo, Control
Journal: bioRxiv
Article Title: Dual effects of nicotinamide on aging-related arrhythmia: protective at low dose, proarrhythmic at high doses
doi: 10.1101/2025.09.22.677785
Figure Lengend Snippet: (A) Experimental scheme of Langendorff ex vivo mouse hearts perfused with 0 mM NAM (control) or 100 mM NAM-supplemented buffer. (B) Representative electrocardiogram (ECG) shows that acute perfusion with 100 mM NAM rapidly induced VF, contractile dysfunction, and eventually led to cardiac arrest (n=4 males, 2 females). (C) Representiative blots and (D) quantification of acetylated SERCA2a levels of left venticular tissue from control and 100 mM NAM-perfused ex vivo mouse hearts (n=3). Data are expressed as mean ± standard error of the mean. Two tailed unpaired Student t-test was used.
Article Snippet:
Techniques: Ex Vivo, Control, Two Tailed Test